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Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine
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神经科学
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JoVE 杂志 神经科学
Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

DOI:
10.3791/60886-v

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06:40 min

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April 23, 2020

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, , ,
1Department of Psychology,Elizabethtown College, 2Department of Psychiatry,Yale University, 3Department of Cellular and Molecular Physiology,Yale University

Chapters

  • 00:04Introduction
  • 00:56Electrode Implantation
  • 03:12Carbon Fiber and Stimulating Electrode/Guide Cannula Location Optimization
  • 04:26Combination Infusion and Stimulation Fast-Scan Cyclic Voltammetry (FSCV) Recording
  • 05:18Results: Representative Analyses of Stimulation-Evoked Phasic Dopamine Release
  • 06:03Conclusion

Summary

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The goal of this protocol is to directly manipulate ventral tegmental area receptors to study their contribution to subsecond dopamine release.

Tags

Ventral Tegmental AreaPhasic Dopamine ReleaseNucleus AccumbensReceptor FunctionCarbon Fiber MicroelectrodeBipolar StimulatorReference ElectrodeSterilizedLambdaBregmaScrewDuraBipolar Stimulating Electrode

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