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Applicazione di Live Cell Imaging e tomografia Cryo-Electron a risolvere le caratteristiche spatiotemporali del sistema di secrezione Legionella pneumophila Dot/Icm
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JoVE Revista Inmunología y contagio
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
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Applicazione di Live Cell Imaging e tomografia Cryo-Electron a risolvere le caratteristiche spatiotemporali del sistema di secrezione Legionella pneumophila Dot/Icm

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09:12 min

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March 10, 2020

DOI:

10.3791/60693-v

DOI:
10.3791/60693-v

•

09:12 min
March 10, 2020

•

3 Views
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1Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine,Yale University School of Medicine, 2Microbial Sciences Institute,Yale University, 3Department of Microbiology and Molecular Genetics, McGovern Medical School,The University of Texas Health Science Center at Houston

Tags

Live Cell ImagingCryo-electron TomographyLegionella PneumophilaDot/Icm Secretion SystemBacterial Secretion ComplexesHost-pathogen InteractionsStructural-functional RelationshipsAgarose PadsFluorescence MicroscopyPolarity Quantification

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Cite this Article

Chetrit, D., Park, D., Hu, B., Liu, J., Roy, C. R. Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System. J. Vis. Exp. (157), e60693, doi:10.3791/60693 (2020).

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