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通过低温BN-MS分析进行高分辨率复杂分析
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Bioquímica
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JoVE Revista Bioquímica
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
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通过低温BN-MS分析进行高分辨率复杂分析

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09:33 min

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October 15, 2019

DOI:

10.3791/60096-v

DOI:
10.3791/60096-v

•

09:33 min
October 15, 2019

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1Institute of Physiology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, 2Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, 3Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, 4Logopharm GmbH, Germany

Tags

CsBN-MSProtein ComplexesMembrane ProteinsSignal TransductionNative Protein ComplexSubunit CompositionGradient GelPolymerizationLoading SlotsRunning BuffersProtein Solubilization

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Müller, C. S., Bildl, W., Klugbauer, N., Haupt, A., Fakler, B., Schulte, U. High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis. J. Vis. Exp. (152), e60096, doi:10.3791/60096 (2019).

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