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Ex Vivo Transmigration Sisteminde Lenfosit Göçünün Değerlendirilmesi
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
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Ex Vivo Transmigration Sisteminde Lenfosit Göçünün Değerlendirilmesi

6,639 Views

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10:25 min

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September 20, 2019

DOI:

10.3791/60060-v

DOI:
10.3791/60060-v

•

10:25 min
September 20, 2019

•

1 Views
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1Department of Internal Medicine,University of Utah, 2Research Service,VA Nebraska Iowa Health Care System, 3Department of Internal Medicine,University of Nebraska Medical Center, 4Department of Environmental, Agricultural & Occupational Health,University of Nebraska Medical Center

Summary

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Bu protokolde lenfositler, alt odadan gözenekli bir zarla ayrılan bir transmigration sisteminin üst odasına yerleştirilir. Keokin alt hazneye eklenir, bu da bir kemokin gradyan boyunca aktif göçe neden olur. 48 saat sonra, lenfositler transmigration quantitate için her iki odada sayılır.

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Cite this Article

Warren, K. J., Wyatt, T. A. Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System. J. Vis. Exp. (151), e60060, doi:10.3791/60060 (2019).

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