Journal
/
Biología del desarrollo
/
An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

5,650 Views

•

08:02 min

•

October 19, 2019

DOI:

10.3791/60031-v

DOI:
10.3791/60031-v

•

08:02 min
October 19, 2019

•

11 Views
, , ,
1School of Life Sciences,The Chinese University of Hong Kong, Hong Kong Special Administrative Region, 2Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences, Shenzhen

Summary

Automatically generated

In this report, we describe a simple protocol for studying neurite outgrowth in embryonic rat cortical neurons by co-transfecting with EGFP and the protein of interest.

Explore More Videos

EGFPNeurite OutgrowthPrimary NeuronsTransfectionNeural RegenerationBrain TraumaNeurodegenerative DiseasesPoly-D-LysineSprague Dawley RatCerebral HemispheresCortexTrypsin EDTAMaintenance Medium

Read Article

Cite this Article

Chan, W. W. R., Li, W., Chau, D. L. D., Lau, K. An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons. J. Vis. Exp. (152), e60031, doi:10.3791/60031 (2019).

Download .ris file

Copy

Share Video

.

Copy