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An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons
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发育生物学
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JoVE 杂志 发育生物学
An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

5,649 Views

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08:02 min

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October 19, 2019

DOI:

10.3791/60031-v

DOI:
10.3791/60031-v

•

08:02 min
October 19, 2019

•

11 Views
, , ,
1School of Life Sciences,The Chinese University of Hong Kong, Hong Kong Special Administrative Region, 2Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences, Shenzhen

Summary

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In this report, we describe a simple protocol for studying neurite outgrowth in embryonic rat cortical neurons by co-transfecting with EGFP and the protein of interest.

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Cite this Article

Chan, W. W. R., Li, W., Chau, D. L. D., Lau, K. An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons. J. Vis. Exp. (152), e60031, doi:10.3791/60031 (2019).

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