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Verwendung von humaninduzierten pluripotenten Stammzellen zur Erzeugung von Tumorantigen-spezifischen T-Zellen
JoVE Revista
Investigación sobre el cáncer
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JoVE Revista Investigación sobre el cáncer
Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
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Verwendung von humaninduzierten pluripotenten Stammzellen zur Erzeugung von Tumorantigen-spezifischen T-Zellen

9,872 Views

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10:57 min

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October 24, 2019

DOI:

10.3791/59997-v

DOI:
10.3791/59997-v

•

10:57 min
October 24, 2019

•

5 Views
, , , , , ,
1Surgery Branch,National Cancer Institute, NIH, 2Center for Cell-Based Therapy,National Cancer Institute, NIH, 3Laboratory of Immunology, Institute for Frontier Life and Medical Sciences,Kyoto University

Summary

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Dieser Artikel beschreibt eine Methode zur Erzeugung funktioneller Tumorantigen-spezifischer induzierter pluripotenter Stammzell-abgeleiteterCD8- + einzelner positiver T-Zellen mit OP9/DLL1-Kokultursystem.

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Cite this Article

Good, M. L., Vizcardo, R., Maeda, T., Tamaoki, N., Malekzadeh, P., Kawamoto, H., Restifo, N. P. Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells. J. Vis. Exp. (152), e59997, doi:10.3791/59997 (2019).

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