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基于原生自荧光的sFLIM测量植物部分荧光探针和木质素之间的相互作用
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Bioquímica
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JoVE Revista Bioquímica
Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
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基于原生自荧光的sFLIM测量植物部分荧光探针和木质素之间的相互作用

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00:07 min

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January 02, 2020

DOI:

10.3791/59925-v

DOI:
10.3791/59925-v

•

00:07 min
January 02, 2020

•

2 Views
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1PICT Platform,Université de Reims Champagne-Ardenne, 2Fractionation of AgroResources and Environment (FARE) Laboratory, INRA,Université de Reims Champagne-Ardenne, 3Unité de Glycobiologie Structurale et Fonctionnelle,Université de Lille

Summary

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该协议描述了结合光谱和荧光寿命测量的原始设置，以评估在厚植物部分直接测定基于红胺的荧光探针和木质素聚合物之间的Fürster共振能量转移（FRET）。

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Terryn, C., Habrant, A., Paës, G., Spriet, C. Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence. J. Vis. Exp. (155), e59925, doi:10.3791/59925 (2020).

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