Journal
/
Inmunología y contagio
/
Fırça hücrelerinin Mouse tracheas 'tan izolasyon ve nicel değerlendirilmesi
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
Please note that all translations are automatically generated. Click here for the English version.

Fırça hücrelerinin Mouse tracheas 'tan izolasyon ve nicel değerlendirilmesi

6,992 Views

•

10:25 min

•

June 12, 2019

DOI:

10.3791/59496-v

DOI:
10.3791/59496-v

•

10:25 min
June 12, 2019

•

3 Views
, , ,
1Division of Rheumatology, Allergy and Immunology, Brigham and Women’s Hospital,Harvard Medical School

Tags

Tracheal Brush CellsEpithelial IsolationPapain DigestionCholine AcetyltransferaseFluorescent Reporter MiceTranscriptional AnalysisRNA SequencingUpper Airway EpitheliumDispaseDNase ITyrode’s BufferFACS BufferEuthanasia

Read Article

Cite this Article

Ualiyeva, S., Yoshimoto, E., Barrett, N. A., Bankova, L. G. Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas. J. Vis. Exp. (148), e59496, doi:10.3791/59496 (2019).

Download .ris file

Copy

Share Video

.

Copy