/

Inmunología y contagio

/

Isolamento e valutazione quantitativa delle celle pennello dalle trachea del topo

JoVE Revista
Inmunología y contagio

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Revista Inmunología y contagio

Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

Please note that all translations are automatically generated. Click here for the English version.

Isolamento e valutazione quantitativa delle celle pennello dalle trachea del topo

6,992 Views

•

10:25 min

•

June 12, 2019

DOI:

10.3791/59496-v

DOI:

10.3791/59496-v

•

10:25 min

June 12, 2019

•

3 Views

Saltanat Ualiyeva, Eri Yoshimoto, Nora A. Barrett, Lora G. Bankova

¹Division of Rheumatology, Allergy and Immunology, Brigham and Women’s Hospital,Harvard Medical School

Tags

Tracheal Brush Cells Epithelial Isolation Papain Digestion Choline Acetyltransferase Fluorescent Reporter Mice Transcriptional Analysis RNA Sequencing Upper Airway Epithelium Dispase DNase I Tyrode’s Buffer FACS Buffer Euthanasia

Read Article