Journal
/
Bioquímica
/
果蝇梅拉诺加斯特的锡酸盐合成酶活性的色度测定
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster
Please note that all translations are automatically generated. Click here for the English version.

果蝇梅拉诺加斯特的锡酸盐合成酶活性的色度测定

DOI:
10.3791/59454-v

•

04:30 min

•

January 16, 2020

•

, , ,
1Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 2Department of Anatomy of Physiology,Shanghai Jiao Tong University School of Medicine

Capítulos

  • 00:04Título
  • 00:38Colorimetric Citrate Synthase Activity Assay
  • 02:46Results: Effect of dRNF34 and dPGC-1 Knockdown on Citrate Synthase Activity
  • 03:38Conclusion

Summary

Traducción Automática

Traducción Automática

我们提出了一种对胆酸合成酶活性的色度测定方案，用于定量果蝇组织均质中完整的线粒体质量。

Tags

Citrate SynthaseColorimetric AssayDrosophila MelanogasterMitochondrial FunctionExtraction BufferProtein ConcentrationEnzymatic ActivityDRNF34DPGC-1

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

的pH依赖性活性的检测<em>大肠杆菌</em>陪护HdeB<em>体外</em>和<em>在体内</em

磷脂Scramblase活动的基于荧光的测定法

测定植物铁含量的比色法

体外检测 tRNA-Isopentenyl 转移酶活性的实验研究

植物细胞悬浮液中咖啡因的提取、酶活性及咖啡因合成酶基因的表达

一种用于蛋白质溶解活性的荧光肽裂解检测: 冠状病毒穗蛋白活化的应用

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

基于荧光的连续核酸内切酶偶联DNA甲基化测定，用于筛选DNA甲基转移酶抑制剂

使用荧光测定法或流式细胞术测量半胱天冬酶活性

RGBradford：使用智能手机相机进行蛋白质定量

Read Article