Journal
/
Biochemie
/
A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

DOI:
10.3791/59454-v

•

04:30 min

•

January 16, 2020

•

, , ,
1Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 2Department of Anatomy of Physiology,Shanghai Jiao Tong University School of Medicine

Kapitel

  • 00:04Titel
  • 00:38Colorimetric Citrate Synthase Activity Assay
  • 02:46Results: Effect of dRNF34 and dPGC-1 Knockdown on Citrate Synthase Activity
  • 03:38Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We present a protocol for a colorimetric assay of citrate synthase activity for quantification of intact mitochondrial mass in Drosophila tissue homogenates.

Tags

Citrate SynthaseColorimetric AssayDrosophila MelanogasterMitochondrial FunctionExtraction BufferProtein ConcentrationEnzymatic ActivityDRNF34DPGC-1

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

A Fluorescence-based Assay of Phospholipid Scramblase Activity

A Colorimetric Method for Measuring Iron Content in Plants

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Author Spotlight: An Alternative Approach to Protein Quantification by Bradford Assay Using a Smartphone

Read Article