Journal
/
生物化学
/
A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

9,660 Views

•

04:30 min

•

January 16, 2020

DOI:

10.3791/59454-v

DOI:
10.3791/59454-v

•

04:30 min
January 16, 2020

•

3 Views
, , ,
1Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 2Department of Anatomy of Physiology,Shanghai Jiao Tong University School of Medicine

Summary

Automatically generated

We present a protocol for a colorimetric assay of citrate synthase activity for quantification of intact mitochondrial mass in Drosophila tissue homogenates.

Explore More Videos

Citrate SynthaseColorimetric AssayDrosophila MelanogasterMitochondrial FunctionExtraction BufferProtein ConcentrationEnzymatic ActivityDRNF34DPGC-1

Read Article

Cite this Article

Wei, P., Liu, Q., Xue, W., Wang, J. A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster. J. Vis. Exp. (155), e59454, doi:10.3791/59454 (2020).

Download .ris file

Copy

Share Video

.

Copy