Journal
/
Genética
/
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
JoVE Revista
Genética
This content is Free Access.
JoVE Revista Genética
Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

DOI:
10.3791/59377-v

•

11:26 min

•

June 03, 2019

•

, , ,
1Institute of Molecular Virology and Cell Biology,Friedrich-Loeffler-Institut, 2Institute of Novel and Emerging Infectious Diseases,Friedrich-Loeffler-Institut, 3Department of Animal Science,Njala University

Capítulos

  • 00:04Título
  • 00:54Amplification of the NPC1 Open Reading Frame
  • 03:36Library Preparation
  • 05:48Quality Check of Flow Cell
  • 06:31Loading the Flow Cell and Starting the Sequencing Run
  • 08:59Results: Analysis of Sequenced mRNA
  • 10:30Conclusion

Summary

Traducción Automática

Traducción Automática

Nanopore sequencing is a novel technology that allows cost-effective sequencing in remote locations and resource-poor settings. Here, we present a protocol for sequencing of mRNAs from whole blood that is compatible with such conditions.

Tags

Keywords: MRNA SequencingNanopore SequencingThird-generation SequencingField LaboratoriesEbola VirusTouchdown PCRCDNA AmplificationMagnetic Bead PurificationEthanol WashLibrary Preparation

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/59377?article_type=v

Methyl-binding DNA capture Sequencing for Patient Tissues
check_url/es/59377?article_type=v

Detection of Copy Number Alterations Using Single Cell Sequencing
check_url/es/59377?article_type=v

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
check_url/es/59377?article_type=v

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
check_url/es/59377?article_type=v

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
check_url/es/59377?article_type=v

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
check_url/es/59377?article_type=v

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
check_url/es/59377?article_type=v

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
check_url/es/59377?article_type=v

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
check_url/es/59377?article_type=v

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Read Article