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In vitro-Generation der Somiten Derivate aus menschlichen induzierten Zellen pluripotenten Stammzellen
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
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In vitro-Generation der Somiten Derivate aus menschlichen induzierten Zellen pluripotenten Stammzellen

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06:21 min

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April 25, 2019

DOI:

10.3791/59359-v

DOI:
10.3791/59359-v

•

06:21 min
April 25, 2019

•

3 Views
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1Department of Life Science Frontiers, Center for iPS Cells Research and Application,Kyoto University, 2Department of Clinical Application, Center for iPS Cells Research and Application,Kyoto University

Summary

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Wir stellen Ihnen hier ein Protokoll für die Differenzierung der menschlichen induzierten pluripotenten Stammzellen in jeder Somiten Derivat (Myotom, Sklerotom Dermatome und Syndetome) in chemisch definierten Bedingungen, die hat Anwendungen in zukünftigen Krankheit Modellierung und zellbasierte Therapien in der orthopädischen Chirurgie.

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Cite this Article

Nakajima, T., Sakurai, H., Ikeya, M. In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (146), e59359, doi:10.3791/59359 (2019).

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