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A Galleria mellonella Oral Administration Model to Study Commensal-Induced Innate Immune Responses
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
A Galleria mellonella Oral Administration Model to Study Commensal-Induced Innate Immune Responses

A Galleria mellonella Oral Administration Model to Study Commensal-Induced Innate Immune Responses

DOI:

06:32 min

March 21, 2019

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Capítulos

  • 00:04Título
  • 00:28G. mellonella Rearing and Larva Preparation and Force Feeding
  • 01:53Orally-Administered Larva Processing and RNA Isolation
  • 04:22Results: Representative Commensal-Induced Gene and Protein Expression in G. mellonella
  • 05:33Conclusion

Summary

Traducción Automática

Here, we provide a detailed protocol for an oral administration model using Galleria mellonella larvae and how to characterize induced innate immune responses. Using this protocol, researchers without practical experience will be able to use the G. mellonella force-feeding method.

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