Journal
/
Biología del desarrollo
/
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

DOI:
10.3791/58975-v

•

07:37 min

•

June 11, 2019

•

, ,
1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute,Stanford University School of Medicine, 2Department of Developmental Biology,Stanford University School of Medicine

Capítulos

  • 00:04Título
  • 00:45Preparation of Differentiation Media
  • 02:04Seed hPSCs onto Plates at Defined Densities for Differentiation
  • 04:39Differentiation of hPSCs into Endodermal Cells and Liver Progenitors
  • 05:37Results: Differentiating Human Pluripotent Stem Cells into Liver Cells
  • 06:58Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol details a monolayer, serum-free method to efficiently generate hepatocyte-like cells from human pluripotent stem cells (hPSCs) in 18 days. This entails six steps as hPSCs sequentially differentiate into intermediate cell-types such as the primitive streak, definitive endoderm, posterior foregut and liver bud progenitors before forming hepatocyte-like cells.

Tags

Human Pluripotent Stem CellsLiver CellsDifferentiationRegenerative MedicineLiver Bud ProgenitorsHepatocyte-like CellsDevelopmental Signaling PathwaysHigh PurityIMDMPVACDM2CDM3CDM4CDM5Small MoleculesGrowth FactorsMatrixHPSCDissociation Agent

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Read Article