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JoVE Journal Biochemistry
Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
 

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Article DOI: 10.3791/58820-v • 09:52 min • January 31st, 2019
January 31st, 2019 •
1, 1, 1, 1
1Department of Pharmaceutical Sciences, University of Nebraska Medical Center

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Here, we present a protocol to characterize nucleosome particles at the single-molecule level using static and time-lapse atomic force microscopy (AFM) imaging techniques. The surface functionalization method described allows for the capture of the structure and dynamics of nucleosomes in high-resolution at the nanoscale.

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Keywords: Atomic Force Microscopy Nucleosome Structure Single-molecule Imaging Protein-DNA Complexes Amyloid Aggregates Alzheimer's Disease Parkinson's Disease Mica Surface Preparation APS Modification Static AFM Imaging
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