Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
Kapitel
- 00:04Titel
- 01:06Functionalization of Mica Surface for Static AFM Imaging of Nucleosomes
- 02:37Preparation of Nucleosome Samples on APS-Mica for Static AFM Imaging
- 03:39Static AFM Imaging of Nucleosomes
- 04:42High-Speed Time-Lapse AFM Imaging of Nucleosome Dynamics
- 07:42Results: Probing the Structure and Dynamics of Nucleosomes
- 09:29Conclusion
Here, we present a protocol to characterize nucleosome particles at the single-molecule level using static and time-lapse atomic force microscopy (AFM) imaging techniques. The surface functionalization method described allows for the capture of the structure and dynamics of nucleosomes in high-resolution at the nanoscale.
