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Genetics

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JoVE Journal Genetics
Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
 

Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells

Article DOI: 10.3791/58790-v • 09:13 min • January 17th, 2019
January 17th, 2019 •
1, 1, 1,2, 1
1Laboratory of Cell Biology and Molecular Genetics, Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, 2Department of Life Sciences, University of Trieste

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Here, we present a protocol to generate cancer cell clones containing a MS2 sequence tag at a single subtelomere. This approach, relying on the MS2-GFP system, enables visualization of the endogenous transcripts of telomeric repeat-containing RNA (TERRA) expressed from a single telomere in living cells.

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Cancer Cell Clones Telomeric Repeat-containing RNA TERRA Telomeres Visualize Living Cells Yeast Telomeric Noncoding RNA TERRA Single Telomere Translocation Technique Researchers Molecules Claudio Oss Pegorar Nicole Bettin Graduate Students AGS Cells SgRNA Cas9 Expressing Vector MS2 Cassette Neomycin Tripsyn Microscope Marker Clones PBS Trypsin
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