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Génération de Clones de cellules de Cancer de visualiser contenant répétition télomérique RNA TERRA exprimée à partir d’un seul télomères dans les cellules vivantes
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Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
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Génération de Clones de cellules de Cancer de visualiser contenant répétition télomérique RNA TERRA exprimée à partir d’un seul télomères dans les cellules vivantes

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09:13 min

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January 17, 2019

DOI:

10.3791/58790-v

DOI:
10.3791/58790-v

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09:13 min
January 17, 2019

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1 Views
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1Laboratory of Cell Biology and Molecular Genetics, Department of Cellular, Computational and Integrative Biology – CIBIO,University of Trento, 2Department of Life Sciences,University of Trieste

Tags

Cancer Cell ClonesTelomeric Repeat-containing RNA (TERRA)Yeast Telomeric Noncoding RNATelomereCell VisualizationSingle TelomereCell CultureCRISPR-Cas9Cell CloningCell FreezingFluorescence Microscopy

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Avogaro, L., Oss Pegorar, C., Bettin, N., Cusanelli, E. Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells. J. Vis. Exp. (143), e58790, doi:10.3791/58790 (2019).

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