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Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
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Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
Capítulos
- 00:04Título
- 00:36Human Whole Blood Extracellular Vesicle (EV) Isolation
- 01:55Sample Staining
- 03:16Sample Analysis
- 06:41Results: Representative Results of Exosome Characterization
- 08:21Conclusion
In this protocol, we describe the complete workflow for rapid isolation of extracellular vesicles from human whole blood and characterization of specific markers by fluorescence-based nanoparticle-tracking analysis. The presented results show a high level of reproducibility and can be adjusted to cell culture supernatants.
