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Using High Content Imaging to Quantify Target Engagement in Adherent Cells
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JoVE Revista Bioquímica
Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

8,426 Views

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07:23 min

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November 29, 2018

DOI:

10.3791/58670-v

DOI:
10.3791/58670-v

•

07:23 min
November 29, 2018

•

4 Views
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1Chemical Biology Consortium Sweden, Science for Life Laboratory,Karolinska Institutet, Solna, 2Department of Medical Biochemistry and Biophysics,Karolinska Institutet, Solna, 3Science for Life Laboratory, Department of Oncology-Pathology,Karolinska Institutet, Stockholm

Summary

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Measurements of drug target engagement are central to effective drug development and chemical probe validation. Here, we detail a protocol for measuring drug-target engagement using high content imaging in a microplate-compatible adaption of the cellular thermal shift assay (CETSA).

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Axelsson, H., Almqvist, H., Seashore-Ludlow, B. Using High Content Imaging to Quantify Target Engagement in Adherent Cells. J. Vis. Exp. (141), e58670, doi:10.3791/58670 (2018).

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