Journal
/
生物化学
/
Using High Content Imaging to Quantify Target Engagement in Adherent Cells
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

8,426 Views

•

07:23 min

•

November 29, 2018

DOI:

10.3791/58670-v

DOI:
10.3791/58670-v

•

07:23 min
November 29, 2018

•

4 Views
, ,
1Chemical Biology Consortium Sweden, Science for Life Laboratory,Karolinska Institutet, Solna, 2Department of Medical Biochemistry and Biophysics,Karolinska Institutet, Solna, 3Science for Life Laboratory, Department of Oncology-Pathology,Karolinska Institutet, Stockholm

Summary

Automatically generated

Measurements of drug target engagement are central to effective drug development and chemical probe validation. Here, we detail a protocol for measuring drug-target engagement using high content imaging in a microplate-compatible adaption of the cellular thermal shift assay (CETSA).

Explore More Videos

High Content ImagingTarget EngagementAdherent CellsChemical BiologyDrug DiscoveryCell LinesPrimary CulturesCoculturesA-431 CellsTrypsinPlate WashingCompound TreatmentHeat ChallengeWater Bath

Read Article

Cite this Article

Axelsson, H., Almqvist, H., Seashore-Ludlow, B. Using High Content Imaging to Quantify Target Engagement in Adherent Cells. J. Vis. Exp. (141), e58670, doi:10.3791/58670 (2018).

Download .ris file

Copy

Share Video

.

Copy