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Biochemistry

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JoVE Journal Biochemistry
Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
 

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Article DOI: 10.3791/58369-v • 08:12 min • September 28th, 2018
September 28th, 2018 •
1, 1, 1
1Molecular Plant Biology, Department of Biochemistry, University of Turku

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A protocol for the elucidation of plant thylakoid protein complex organization and composition with blue native polyacrylamide gel electrophoresis (BN-PAGE) and 2D-SDS-PAGE is described. The protocol is optimized for Arabidopsis thaliana, but can be used for other plant species with minor modifications.

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Keywords: Thylakoid Membrane Protein Complexes Blue Native Gel Electrophoresis Photosynthetic Protein Complexes Protein Complex Organization Gradient Gel Acrylamide Solutions Polymerization Stacking Gel
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