/
/
Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes
This content is Free Access.
Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes
12,780 Views
•
11:25 min
•
May 19, 2019
DOI:
Summary
Automatically generated
Here we present a protocol to extract, resolve and identify mitochondrial supercomplexes which minimizes exposure to detergents and Coomassie Blue. This protocol offers an optimal balance between resolution, and preservation of enzyme activities, while minimizing the risk of losing labile protein-protein interactions.
Read Article
Cite this Article
Cuillerier, A., Burelle, Y. Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes. J. Vis. Exp. (147), e59294, doi:10.3791/59294 (2019).
Copy