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Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
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Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
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06:36 min
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July 28, 2017
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Summary
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Native polyacrylamide gel electrophoresis is a fundamental tool for analyzing RNA-protein interactions. Traditionally most experiments have used vertical gels. However, horizontal gels provide several advantages, such as the opportunity to monitor complexes during electrophoresis. We provide a detailed protocol for generating and using horizontal native gel electrophoresis.
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Dowdle, M. E., Imboden, S. B., Park, S., Ryder, S. P., Sheets, M. D. Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes. J. Vis. Exp. (125), e56031, doi:10.3791/56031 (2017).
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