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כימות של Efferocytosis על-ידי קרינה פלואורסצנטית תא בודד מיקרוסקופ
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
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כימות של Efferocytosis על-ידי קרינה פלואורסצנטית תא בודד מיקרוסקופ

12,137 Views

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06:15 min

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August 18, 2018

DOI:

10.3791/58149-v

DOI:
10.3791/58149-v

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06:15 min
August 18, 2018

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2 Views
, , ,
1Department of Microbiology and Immunology and the Center for Human Immunology,University of Western Ontario, 2Institute of Infection and Global Health,University of Liverpool, 3Department of Respiratory Research,Aintree University Hospital NHS Foundation Trust

Tags

EfferocytosisSingle-cell Fluorescence MicroscopyApoptotic Cell UptakeMacrophagesJurkat CellsNHS-BiotinCell-tracking DyeFITC-conjugated StreptavidinQuantificationMicroscope Optimization

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Taruc, K., Yin, C., Wootton, D. G., Heit, B. Quantification of Efferocytosis by Single-cell Fluorescence Microscopy. J. Vis. Exp. (138), e58149, doi:10.3791/58149 (2018).

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