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Isolation og fluorescens Imaging for enkelt-partikel genopbygning af Chlamydomonas centrioler
JoVE Revista
Biología
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JoVE Revista Biología
Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Isolation og fluorescens Imaging for enkelt-partikel genopbygning af Chlamydomonas centrioler

DOI:
10.3791/58109-v

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10:38 min

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September 21, 2018

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, , , , ,
1Department of Cell Biology, Sciences III,University of Geneva

Capítulos

  • 00:04Título
  • 00:46Purification of C. reinhardtii Centrioles
  • 05:15Concentration of Centrioles onto the Center of the Coverslips
  • 07:06Quantification of Isolated Centrioles on Coverslips: Centrifugation and Immunofluorescenc
  • 08:47Results: Isolation and Fluorescence Imaging of Chlamydomonas Centrioles
  • 10:03Conclusion

Summary

Traducción Automática

Traducción Automática

Vi har udviklet en strategi for at rense og billed et stort antal af centrioler i forskellige orienteringer modtagelig for Super-resolution mikroskopi og enkelt-partikel gennemsnit.

Tags

CentrioleChlamydomonasSingle-particle ReconstructionFluorescence ImagingIsolationProtein LocalizationDeflagellationSucrose CushionCentrifugationPBSLysis Buffer

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