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衣藻中心粒单粒子重建的分离与荧光成像
JoVE Revista
Biología
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JoVE Revista Biología
Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
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衣藻中心粒单粒子重建的分离与荧光成像

9,183 Views

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10:38 min

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September 21, 2018

DOI:

10.3791/58109-v

DOI:
10.3791/58109-v

•

10:38 min
September 21, 2018

•

4 Views
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1Department of Cell Biology, Sciences III,University of Geneva

Summary

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我们已经制定了一个战略, 以净化和图像大量的中心粒在不同的方向, 适合超分辨率显微镜和单粒子平均。

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CentrioleChlamydomonasSingle-particle ReconstructionFluorescence ImagingIsolationProtein LocalizationDeflagellationSucrose CushionCentrifugationPBSLysis Buffer

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Klena, N., Gambarotto, D., Le Guennec, M., Borgers, S., Guichard, P., Hamel, V. Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles. J. Vis. Exp. (139), e58109, doi:10.3791/58109 (2018).

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