Journal
/
Bioquímica
/
Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

DOI:
10.3791/57949-v

•

06:43 min

•

June 21, 2018

•

, , , ,
1Department of Horticultural Science, Microbial and Plant Genomics Institute,University of Minnesota, 2Department of Horticultural Science, Department of Plant and Microbial Biology, Microbial and Plant Genomics Institute,University of Minnesota, 3Department of Botany and Zoology,Stellenbosch University

Capítulos

  • 00:05Título
  • 00:50Modification and Preparation of the MS System for Leaf Spray MS
  • 01:38Preparation of Instrument, Solvents, and Plant Tissue
  • 03:39Data Quality Assessment and Tandem Mass Fragmentation
  • 05:06Results: Detection of Mesembrine Alkaloids from Sceletium tortuosum
  • 06:19Conclusion

Summary

Traducción Automática

Traducción Automática

Leaf spray mass spectrometry is a direct chemical analysis technique that minimizes the sample preparation and eliminates chromatography, allowing for the rapid detection of small molecules from plant tissues.

Tags

Leaf Spray Mass SpectrometryAmbient IonizationPlant MetabolitesSceletium TortuosumMesembrine AlkaloidsPesticidesSample PreparationNano Spray SourceContaminant IonsData File

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Read Article