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多年生草试验中土壤、根际和根系微生物群落的分离与分析

JoVE Revista
Medio ambiente

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JoVE Revista Medio ambiente

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

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多年生草试验中土壤、根际和根系微生物群落的分离与分析

DOI:

10.3791/57932-v

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10:31 min

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July 24, 2018

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Morgan R. McPherson, Peng Wang, Ellen L. Marsh, Robert B. Mitchell, Daniel P. Schachtman

¹Department of Agronomy and Horticulture and Center for Plant Science Innovation,University of Nebraska-Lincoln, ²USDA-ARS, Wheat, Sorghum and Forage Research Unit,University of Nebraska-Lincoln East Campus

Capítulos

00:04Título
00:36Plant Excavation, Soil Removal, and Bulk Soil Collection
01:46Root and Rhizosphere Collection
02:44Root Surface Sterilization
03:54Processing Rhizosphere Samples
05:02DNA Extraction and Soil Analysis Processing
06:33DNA Extraction in 96-well Format
07:45Rhizosphere Sample Loading
08:47Results: Visualization and Statistical Methods Highlight Differences in Microbial Community Composition and Diversity Between Sample Types and Grass Species
10:03Conclusion

Summary

Traducción Automática

详细描述了植物根系从田间开挖以及样品加工到 endosphere、根际和土壤的情况, 包括 DNA 提取和数据分析方法。本文旨在使其他实验室能够利用这些技术来研究土壤、endosphere 和根际微生物。

Tags

Keywords: Microbial Communities Soil Rhizosphere Root Endosphere Perennial Grass Field Experiments Sample Collection Soil Sampling Root Sampling Rhizosphere Extraction Root Sterilization Microbiome Analysis

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