/

/

HEK 细胞 Mitoribosome 的快速分离

JoVE Revista
Bioquímica

This content is Free Access.

JoVE Revista Bioquímica

Rapid Isolation of the Mitoribosome from HEK Cells

Please note that all translations are automatically generated. Click here for the English version.

HEK 细胞 Mitoribosome 的快速分离

DOI:

10.3791/57877-v

•

09:33 min

•

October 04, 2018

•

Shintaro Aibara, Juni Andréll, Vivek Singh, Alexey Amunts

¹Science for Life Laboratory, Department of Biochemistry and Biophysics,Stockholm University

Capítulos

00:04Título
00:53Mitochondria Isolation
05:15Mitoribosome Purification
08:12Results: Isolated Mitoribosomes from HEK Cells
09:10Conclusion

Summary

Traducción Automática

线粒体有专门的核糖体, 与他们的细菌和细胞质的对应物分叉。在这里, 我们展示如何 mitoribosomes 可以获得从他们的本地舱在 HEK 细胞。该方法包括分离悬浮细胞中的线粒体, 并由此纯化 mitoribosomes。

Tags

Mitoribosome HEK Cells Protein Synthesis Mitochondria Nitrogen Cavitation Cell Lysis High-resolution Structure Cancer Treatment HEK293S Cells PBS MIB Buffer SM4 Buffer Nitrogen Cavitation Chamber Cell Debris Nuclei MiBSM Buffer Teflon Glass Down Homogenizer

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

从细胞使用寡核苷酸定向洗脱同源RNA-蛋白质复合物的隔离

从Cryomilled哺乳动物细胞中的蛋白质复合物亲和捕获

从真核细胞蛋白质复合体串联亲和纯化

生物素化细胞表面蛋白的纯化<emRigicephalus microplus</em>上皮细胞

拟南芥线粒体的分离和呼吸测量

用石英谐振器研究磷脂结合蛋白附件 a2 与固体支持的脂质层结合动力学的耗散微重法

寄生性锥虫锥虫锥虫的 f1-atpase 的分离

组织细胞外囊泡与肝脏分离

翻译核糖体亲性纯化(TRAP),用于从体内内皮细胞中分离RNA

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

Read Article