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Developmental Biology

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JoVE Journal Developmental Biology
Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
 

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

Article DOI: 10.3791/57785-v • 08:42 min • June 26th, 2018
June 26th, 2018 •
*1, *1, 1, 1, 1, 1, 2,3
1Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, 2Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 3School of Doctoral Studies and Research, Universidad Europea de Madrid
* These authors contributed equally

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Here we describe the standard protocol for the detection of β-galactosidase activity in early whole mouse embryos and the method for paraffin sectioning and counterstaining. This is an easy and quick procedure to monitor gene expression during development that can also be applied to tissue sections, organs or cultured cells.

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Gene Expression &946;-galactosidase Activity Mouse Embryos Developmental Biology Gene Expression Patterns Sensitive Technique Quick Procedure Direct Visualization Staining Paraffin Sectioning Transgenic Embryos X-gal Rinse Buffer Negative Controls X-gal Staining Solution Reporter Gene Cell Lineage Studies Hydrolysis Of Substrate X-gal Blue Precipitate
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