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Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

25,371 Views

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08:42 min

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June 26, 2018

DOI:

10.3791/57785-v

DOI:
10.3791/57785-v

•

08:42 min
June 26, 2018

•

14 Views
, , , , , ,
1Faculty of Biomedical and Health Sciences,Universidad Europea de Madrid, 2Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 3School of Doctoral Studies and Research,Universidad Europea de Madrid

Summary

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Here we describe the standard protocol for the detection of β-galactosidase activity in early whole mouse embryos and the method for paraffin sectioning and counterstaining. This is an easy and quick procedure to monitor gene expression during development that can also be applied to tissue sections, organs or cultured cells.

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β-galactosidaseGene ExpressionWhole MountX-gal StainingMouse EmbryosEntwicklungsbiologieReporter GeneLineage TracingHistochemical DetectionParaffin Sectioning

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Cite this Article

Blanco, M. J., Learte, A. I., Marchena, M. A., Muñoz-Sáez, E., Cid, M. A., Rodríguez-Martín, I., Sánchez-Camacho, C. Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos. J. Vis. Exp. (136), e57785, doi:10.3791/57785 (2018).

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