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Split-BioID — Analisi proteomica dei complessi della proteina contesto specifico in ambiente nativo di cellulare
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
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Split-BioID — Analisi proteomica dei complessi della proteina contesto specifico in ambiente nativo di cellulare

19,041 Views

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09:02 min

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April 20, 2018

DOI:

10.3791/57479-v

DOI:
10.3791/57479-v

•

09:02 min
April 20, 2018

•

3 Views
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1Cluster of excellence CellNetworks,Heidelberg University, 2Heidelberg University Biochemistry Center (BZH)

Tags

Split-BioIDProteomic AnalysisProtein ComplexesNative Cellular EnvironmentProtein Fragment Complementation AssayProximity-dependent Protein BiotinylationCell BiologyMass Spectrometric AnalysisStreptavidin PulldownProtein Complexes RemodelingContext-specific Protein Complexes

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Cite this Article

Schopp, I. M., Béthune, J. Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment. J. Vis. Exp. (134), e57479, doi:10.3791/57479 (2018).

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