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Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
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Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
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April 20, 2018
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We provide a step-by-step protocol for split-BioID, a protein fragments-complementation assay based on the proximity-labeling technique BioID. Activated on the interaction of two given proteins, it allows the proteomics analysis of context-dependent protein complexes in their native cellular environment. The method is simple, cost-effective and only requires standard laboratory equipment.
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Schopp, I. M., Béthune, J. Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment. J. Vis. Exp. (134), e57479, doi:10.3791/57479 (2018).
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