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Journal / Developmental Biology / Culturing of Retinal Pigment Epithelial Cells on…
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Developmental Biology

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JoVE Journal Developmental Biology
Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane
 

Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane

Article DOI: 10.3791/57084-v • 08:32 min • April 12th, 2018
April 12th, 2018 •
1, 1, 1, 2, 1
1Department of Ophthalmology and Visual Science, Yale School of Medicine, 2Edward S. Harkness Eye Institute, Columbia University School of Medicine

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The goal of this protocol is to demonstrate the culturing of retinal pigment epithelial (RPE) cells on aged and/or diseased human Bruch's membrane. This method is suitable to study RPE cell behavior on a compromised extracellular matrix.

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Keywords: Retinal Pigment Epithelial Cells Bruch's Membrane Ex Vivo Model Aged Human AMD Etiology Proteomics RPE Cell Culture Sclera Vitreous Retina Optic Nerve Ammonium Hydroxide DPBS PTFE Membrane
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