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Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells
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Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells
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07:48 min
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September 06, 2018
DOI:
1U861, I-Stem, Association Française contre les Myopathies (AFM),Institut National de la Santé et de la Recherche Médicale (INSERM), 2U861, I-Stem, Association Française contre les Myopathies (AFM),Université Evry Val-d’Essonne (UEVE), 3I-Stem, Association Française contre les Myopathies (AFM),Centre pour L’Etude des Cellules Souches (CECS), 4Banque de tissus humain, Hôpital Saint Louis, Assistance Publique – Hôpitaux de Paris (AP-HP), 5Sorbonne Université, INSERM, CNRS, Institut de la Vision, F-75012
Summary
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We describe a method to engineer a retinal tissue composed of retinal pigment epithelial cells derived from human pluripotent stem cells cultured on top of human amniotic membranes and its preparation for grafting in animal models.
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Ben M'Barek, K., Habeler, W., Plancheron, A., Jarraya, M., Goureau, O., Monville, C. Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells. J. Vis. Exp. (139), e58216, doi:10.3791/58216 (2018).
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