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用 5-Bromouridine 标记和免疫沉淀 RNA 合成的研究

JoVE Revista
Genética

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JoVE Revista Genética

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

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用 5-Bromouridine 标记和免疫沉淀 RNA 合成的研究

9,311 Views

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09:59 min

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May 03, 2018

DOI:

10.3791/57056-v

DOI:

10.3791/57056-v

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09:59 min

May 03, 2018

•

2 Views

Rikke H. Kofoed¹, Cristine Betzer¹, Søren Lykke-Andersen², Ewa Molska², Poul H. Jensen¹

¹DANDRITE – Danish Research Institute of Translational Neuroscience, Dept. of Biomedicine,Aarhus University, ²Dept. of Molecular Biology and Genetics,Aarhus University

Summary

该方法可用于测量 RNA 的合成。5-Bromouridine 被添加到细胞中, 并纳入合成 RNA。rna 的合成是通过标记后立即提取 rna 来测量的, 其次是标记 rna 的 5 Bromouridine 靶向免疫沉淀, 并通过反向转录和定量聚合酶链反应进行分析。

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