Journal
/
Neurociencias
/
Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

7,358 Views

•

07:05 min

•

January 19, 2018

DOI:

10.3791/57015-v

DOI:
10.3791/57015-v

•

07:05 min
January 19, 2018

•

1 Views
,
1Department of Genetics and Evolution,University of Geneva

Summary

Automatically generated

The goal of this protocol is to establish ex vivo Drosophila larval brain culture optimized to monitor circadian molecular rhythms with long-term fluorescence time-lapse imaging. The application of this method to pharmacological assays is also discussed.

Explore More Videos

Single-cellFluorescenceLive ImagingDrosophilaCircadian ClocksLarval Brain CultureNeurogeneticsGene ExpressionFluorescent ProbesSAM MediumThrombinPenicillin StreptomycinFibrinogenDissectionForcepsDSSThird Instar LarvaeBrain TransferEmbedding Procedure

Read Article

Cite this Article

Sabado, V., Nagoshi, E. Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture. J. Vis. Exp. (131), e57015, doi:10.3791/57015 (2018).

Download .ris file

Copy

Share Video

.

Copy