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Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture
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神经科学
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JoVE 杂志 神经科学
Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

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07:05 min

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January 19, 2018

DOI:

10.3791/57015-v

DOI:
10.3791/57015-v

•

07:05 min
January 19, 2018

•

1 Views
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1Department of Genetics and Evolution,University of Geneva

Summary

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The goal of this protocol is to establish ex vivo Drosophila larval brain culture optimized to monitor circadian molecular rhythms with long-term fluorescence time-lapse imaging. The application of this method to pharmacological assays is also discussed.

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Cite this Article

Sabado, V., Nagoshi, E. Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture. J. Vis. Exp. (131), e57015, doi:10.3791/57015 (2018).

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