Here we present a protocol for wide-area scanning probe nanolithography enabled by the iterative alignment of probe arrays, as well as the utilization of lithographic patterns for cell-surface interaction studies.
Lee, I., Hosford, J., Wang, S., Hunt, J. A., Curran, J. M., Heath, W. P., Wong, L. S. Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies. J. Vis. Exp. (136), e56967, doi:10.3791/56967 (2018).