This protocol aims to achieve surface engineering of pancreatic islets using a heparin-incorporated starPEG nanocoating via pseudo-bioorthogonal chemistry between the N-hydroxysuccinimide groups of the nanocoating and the amine groups of islet cell membrane.
Yang, J., Lou, S., Kong, D., Li, C. Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating. J. Vis. Exp. (136), e56879, doi:10.3791/56879 (2018).