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Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis
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JoVE Journal Chemistry
Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

DOI:
10.3791/56797-v

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10:50 min

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November 02, 2018

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, , , , ,
1Centre for Biospectroscopy,Monash University, 2Department of Microbiology, Faculty of Medicine, Nursing and Health Sciences,Monash University, 3Centre for Biomedical Research,Burnet Institute, 4Department of Medicine,University of Melbourne

Chapters

  • 00:04Title
  • 01:04Synchronization of Parasites
  • 02:43Preparation of the Parasitemia Dilution Series
  • 04:09Spectral Acquisition
  • 05:28Multivariate Data Analysis (MVDA)
  • 08:36Results: ATR-FTIR Spectroscopy and MVDA Detects and Quantifies Parasites in Aqueous RBCs
  • 09:41Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the detection and quantification of Plasmodium falciparum in infected aqueous red blood cells using an attenuated total reflection infrared spectrometer and multivariate data analysis.

Tags

Keywords: Plasmodium FalciparumATR-FTIR SpectroscopyMalaria ParasitemiaMultivariate Data AnalysisPoint-of-care DiagnosticsPhenotypic ResponseAntimicrobial Agents3D7 StrainSorbitol SynchronizationRed Blood CellsSaline Wash

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