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果蝇蛹卵巢的解剖与染色
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Dissection and Staining of Drosophila Pupal Ovaries
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果蝇蛹卵巢的解剖与染色

DOI:
10.3791/56779-v

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07:35 min

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March 02, 2018

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, ,
1Department of Biological Sciences,Columbia University, 2Department of Cell and Systems Biology,University of Toronto

Capítulos

  • 00:05Título
  • 00:41Egg Laying and Female Larvae Selection
  • 02:12Pupae Antibody Staining Preparation
  • 04:24Immunohistochemistry
  • 05:52Dissecting and Mounting Pupal Ovaries
  • 06:49Results: Clear Antibody Staining that Reveals Drosophila Structure and Cellular Organization
  • 07:12Conclusion

Summary

Traducción Automática

Traducción Automática

果蝇卵巢是研究干细胞小生境发育的优良模型系统。虽然已经公布了解剖幼虫和成人卵巢的方法, 蛹卵巢解剖需要不同的技术, 没有详细发表。在这里, 我们概述了解剖, 染色和安装蛹卵巢的协议。

Tags

DrosophilaPupal OvariesDissectionStainingStem Cell DifferentiationTime Course ExperimentsStem Cell DivisionWandering Third Instar LarvaePhosphate Buffered Saline (PBS)Male And Female LarvaePupariationPre-pupaGlass Pipette TipProtein Glue

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