This protocol describes intratracheal inoculations of Fischer 344 rats with Francisella tularensis. This procedure mimics pulmonary exposure of humans to this potential biothreat agent and can be used to test vaccine and therapeutic efficacy against pulmonary tularemia.
Nguyen, J. Q., Zogaj, X., Adelani, A. A., Chu, P., Yu, J., Arulanandam, B. P., Klose, K. E. Intratracheal Inoculation of Fischer 344 Rats with Francisella tularensis. J. Vis. Exp. (127), e56123, doi:10.3791/56123 (2017).