The goal of this protocol is to isolate endothelial progenitor cells from umbilical cord blood. Some of the applications include using these cells as a biomarker for identifying patients with cardiovascular risk, treating ischemic diseases, and creating tissue-engineered vascular and heart valve constructs.
Ravishankar, P., Zeballos, M. A., Balachandran, K. Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood. J. Vis. Exp. (127), e56021, doi:10.3791/56021 (2017).