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发育生物学
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Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood
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JoVE 杂志 发育生物学
Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood

DOI:
10.3791/56021-v

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07:26 min

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September 14, 2017

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, ,
1Department of Biomedical Engineering,University of Arkansas

Chapters

  • 00:05Title
  • 00:50Cord Blood Endothelial Progenitor Cell Isolation
  • 03:34Endothelial Progenitor Cells Expansion
  • 04:58Results: Representative Cord Blood Endothelial Progenitor Cell Analyses
  • 06:21Conclusion

Summary

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The goal of this protocol is to isolate endothelial progenitor cells from umbilical cord blood. Some of the applications include using these cells as a biomarker for identifying patients with cardiovascular risk, treating ischemic diseases, and creating tissue-engineered vascular and heart valve constructs.

Tags

Keywords: Endothelial Progenitor CellsUmbilical Cord BloodVascular GraftsNeovascularizationCardiovascular DiseaseDensity Gradient CentrifugationMononuclear CellsEndothelial Cell Colonies

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