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Redning og karakterisering af rekombinant virus fra en ny verden Zika Virus infektiøs klon
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

Redning og karakterisering af rekombinant virus fra en ny verden Zika Virus infektiøs klon

DOI:
10.3791/55857-v

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07:35 min

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June 07, 2017

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, , , ,
1Department of Microbiology, Immunology, and Pathology,Colorado State University, 2Division of Vector-Borne Diseases,Centers for Disease Control and Prevention

Capítulos

  • 00:05Título
  • 00:40Preparation of In Vitro Transcribed RNA
  • 02:54Rescue of Infectious Virus by Electroporation
  • 05:56Results: In Vitro and In Vivo Characteristics of Clone-derived Virus
  • 07:02Conclusion

Summary

Traducción Automática

Traducción Automática

Denne protokol beskriver genopretningen af ​​infektiøs Zika-virus fra en to-plasmid-infektiøs cDNA-klon.

Tags

Zika VirusInfectious CloneRecombinant VirusFlavivirusPlasmid DigestionLigationIn Vitro TranscriptionVero CellsElectroporation

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